RESUMO
The objectives of our research were to study the chemical composition and the in vitro anticancer effect of the essential oil of Salvia verbenaca growing in natural sites in comparison with those of cultivated (Sc) plants. The oil from wild (Sw) S. verbenaca presented hexadecanoic acid (23.1%) as the main constituent, while the oil from Sc plants contained high quantities of hexahydrofarnesyl acetone (9.7%), scarce in the natural oil (0.7%). The growth-inhibitory and proapoptotic effects of the essential oils from Sw and Sc S. verbenaca were evaluated in the human melanoma cell line M14, testing cell vitality, cell membrane integrity, genomic DNA fragmentation and caspase-3 activity. Both the essential oils were able to inhibit the growth of the cancer cells examined inducing also apoptotic cell death, but the essential oil from cultivated samples exhibited the major effects.
Assuntos
Antineoplásicos Fitogênicos/química , Óleos Voláteis/química , Óleos de Plantas/química , Salvia/química , Apoptose , Caspase 3/metabolismo , Linhagem Celular Tumoral/efeitos dos fármacos , Fragmentação do DNA , Humanos , Melanoma/patologia , Ácido Palmítico/química , Ácido Palmítico/isolamento & purificação , Terpenos/química , Terpenos/isolamento & purificaçãoRESUMO
Phytochemical investigation of the acetone extract of the aerial parts of Onopordum illyricum L. afforded five known sesquiterpenoids: compounds 3 and 4 already isolated from O. illirycum, and 8alpha-[4'-hydroxymethacryloyloxy]-sonchucarpolide (1), 8alpha-[4'-hydroxymethacryloyloxy]-4-epi-sonchucarpolide (2) and 8-(4'-hydroxymethacryloyl)-dehydromelitensin (5), not previously detected in this species. Compounds 4 and 5 showed moderate antifeedant activity against larvae of Spodoptera littoralis.
Assuntos
Comportamento Alimentar/efeitos dos fármacos , Onopordum/química , Sesquiterpenos/farmacologia , Animais , Larva/efeitos dos fármacos , SpodopteraRESUMO
Salvia verbenaca L. (syn. S. minore) is a perennial herb known in the traditional medicine of Sicily as "spaccapetri" and is used to resolve cases of kidney stones, chewing the fresh leaves or in decoction. The chemical composition of the essential oil obtained from aerial parts of S. verbenaca collected in Piano Battaglia (Sicily) on July 2009, was analyzed by GC and GC-MS. The oil was strongly characterized by fatty acids (39.5%) and carbonylic compounds (21.2%), with hexadecanoic acid (23.1%), (Z)-9-octadecenoic acid (11.1%) and benzaldehyde (7.3%) as the main constituents. The in vitro activity of the essential oil against some microorganisms in comparison with chloramphenicol by the broth dilution method was determined. The oil exhibited a good activity as inhibitor of growth of Gram + bacteria.